We provide
cost effective experimental design and execution of functional genomics projects based on the RIBO-seq platform.
TB-Seq specializes in application and development of experimental and computational procedures for identification and analysis of ribosome-protected mRNA footprints (ribosome profiling), from mammalian cell lines and tissues, plants, fungi, bacterial cultures, and metagenome communities.
Let’s work on your project together
Experimental Design
Since 2015, TB-Seq has provided to its international customers from academia, biotech and pharma the highest quality experimental and computational products in ribosome profiling (RIBO-seq) analysis. We offer competitive pricing, fast turn-around, highest quality of execution, and customized project and experimental design and execution, to fit your needs from data collection, through data analysis, to result summary.
TB-Seq Inc. labs are located in South San Francisco CA, at the core of the San Francisco peninsula Biotech hub and in close proximity to Stanford University and UCSF.
We will be glad to discuss with you your project details.
RIBO-seq library preparation
We are experienced in isolating ribosome-bound mRNA from bacterial and eukaryotic cells, and from animal and plant tissues. We have developed sample-specific efficient protocols for all steps of the RIBO-seq procedure, from lysis to library preparation, that allow us to create RIBO-seq libraries of the highest quality from the smallest amount of starting material.
Our proprietary optimized RIBO-seq protocols allow us to produce RIBO-seq libraries of the highest quality more efficiently and with enhanced sensitivity compared to competing protocols.
Sample requirements
RNA-seq libraries
We will be glad to prepare RNA-seq libraries in parallel to RIBO-seq libraries upon request. Comparing RIBO-seq to RNA-seq coverages allows to normalize ribosome coverage by transcript coverage.
High-Throughput Sequencing
We have partnered with experienced sequencing facilities that guarantee fast sequencing services on Illumina HiSeq or NovaSeq sequencers.
Bioinformatics analysis of NGS data
TB-Seq offers a wide range of bioinformatics analyses based on RIBO-seq and RNA-seq sequencing data, ranging from basic quality-control and gene coverage analysis, to differential gene coverage analysis, ORF analysis of 3-periodicity in coverage, codon occupancy analysis, and more.
TB-Seq offers customized analyses tailored to your research needs, using available software packages, our suite of bioinformatics tools, or developing ad hoc analytical tools.
Our team works side-by-side with all clients to understand their research questions and to ensure alignment to their study objectives.
All information on our ribosome profiling services is subject to change without notice. Please contact TB-SEQ Inc. for latest pricing and terms.